optimization of incubation temperature in embryonated chicken eggs inoculated with h9n2 vaccinal subtype of avian influenza virus

Optimization of incubation temperature in embryonated chicken eggs inoculated with H9N2 vaccinal subtype of avian influenza virus

There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into ...

Optimization of incubation temperature in embryonated chicken eggs inoculated with H9N2 vaccinal subtype of avian influenza virus

There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10(-5) dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-d...

Monitoring virus harvesting time in embryonated chicken eggs inoculated with avian influenza H9N2 vaccine strain

Knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. The present study aimed to evaluate the best harvesting time of H9N2 avian influenza virus (AIV) vaccine strain inoculated in specific pathogen free (SPF) embryonated chicken eggs (ECE)s. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated into 336, 11-day old ECE...

monitoring virus harvesting time in embryonated chicken eggs inoculated with avian influenza h9n2 vaccine strain

knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old ece...

Evolution of H9N2 avian influenza virus in embryonated chicken eggs with or without homologous vaccine antibodies

BACKGROUND Vaccines constitute a unique selective pressure, different from natural selection, drives the evolution of influenza virus. In this study, A/Chicken/Shanghai/F/1998 (H9N2) was continually passaged in specific pathogen-free embryonated chicken eggs with or without selective pressures from antibodies induced by homologous maternal antibodies. Genetic mutations, antigenic drift, replica...

Isolation and Identification of Avian Influenza Virus H9N2 Subtype